Table 2. Fluorescence quenching parameters of wild-type (WT) CYP2D6, W128F mutant CYP2D6, MAMC alone and MAMC in the presence of the enzymes, obtained from (linear) Stern–Volmer plots.
Ksv, quenching constant; kq, quenching rate; τav, average fluorescence lifetime; fa, accessible fraction.
| Acrylamide | Iodide | |||||||
|---|---|---|---|---|---|---|---|---|
| KSV (M−1) | kq (109×M−1·s−1) | τav (ns) | fa | kq (M−1) | (109×M−1·s−1) | τav (ns) | fa | |
| WT | 4.5 | 12.5 | 0.36 | 1.1 | 0.7 | 1.8 | 0.40 | 0.3 |
| WT+MAMC | 3.8 | 9.7 | 0.39 | 0.8 | 0.6 | 1.5 | 0.40 | 0.3 |
| W128F | 3.9 | 13.0 | 0.30 | 1.1 | 0.9 | 3.0 | 0.30 | 0.4 |
| W128F+MAMC | 4.1 | 10.5 | 0.39 | 1.0 | 0.8 | 2.1 | 0.39 | 0.3 |
| MAMC | 2.7 | 1.5 | 1.76 | 0.9 | 14.1 | 17.2 | 0.82 | 1.1 |
| MAMC+WT | 3.1 | 1.9 | 1.55 | 0.8 | 15.2 | 10.3 | 1.47 | 1.1 |
| MAMC+W128F | 2.9 | 1.8 | 1.59 | 0.9 | 18.4 | 11.6 | 1.59 | 1.1 |