Skip to main content
. 2006 Jan 13;393(Pt 3):657–668. doi: 10.1042/BJ20050791

Figure 1. LPA stimulates IL-8 gene expression and secretion in HBEpCs.

Figure 1

(A) HBEpCs (approx. 80% confluence in 35 mm dishes) were treated with BEGM containing 0.1% (w/v) BSA (vehicle) or BEGM containing 0.1% (w/v) BSA plus LPA (1 μM) for 30–180 min. IL-8 gene expression was evaluated by real-time RT-PCR as described in the Experimental procedures section. Results are representative of at least three independent experiments. (B) HBEpCs (approx. 80% confluence in 35 mm dishes) were treated with BEGM containing 0.1% (w/v) BSA (vehicle) or BEGM containing 0.1% (w/v) BSA plus LPA (1 μM) for 1–24 h. IL-8 in the medium was quantified by ELISA as described in the Experimental procedures section. The results are expressed as the means±S.D. (n=3) performed in triplicate. *, statistically different from control (P<0.05).