FIG. 5.

(A) LMP-1 induced the TRAF1 protein (arrow) in BJA-B cells but not in CEM cells. Whole-cell lysates (40 μg) from LMP-expressing and control vector-transfected cells were resolved by SDS-10% PAGE. After transfer onto nylon membranes, TRAF1 was incubated with anti-TRAF1 antibody followed by detection with alkaline phosphatase-conjugated anti-rabbit antibody. The lanes represent different clones (the same as lanes 1 to 9 of Fig. 3). (B) LMP-1 (arrows) interacted with TRAF1 in BJA-B cells but not in CEM cells. TRAF1 was immunoprecipitated from cytoplasmic fractions (a) and whole-cell lysates (b), resolved by SDS-PAGE, and electroblotted onto nylon membranes. The membranes were probed for LMP-1 with LMP-specific monoclonal antibodies (CS1-4) and alkaline phosphatase-conjugated secondary antibodies. Lane 1 represents immunoprecipitation with normal rabbit serum. Lanes 2 to 10 in panel B correspond to lanes 1 to 9 in panel A, respectively.