TABLE 1.
Effects of LMP-1 expression on cell cycle progression
| Cell line | % of cells in cell cycle stagea
|
G2/G1 ratio | ||
|---|---|---|---|---|
| G0/G1 | S | G2/M | ||
| BJA-B IgNEO | 29.26 | 66.52 | 3.86 | 1.80 |
| BJA-B IgLMP-1(A) | 34.39 | 46.91 | 18.70 | 1.86 |
| BJA-B IgLMP-1(B) | 32.51 | 48.81 | 18.68 | 1.85 |
| BJA-B IgLMP-1(C) | 39.36 | 47.41 | 12.63 | 1.87 |
| CEM IgNEO | 55.46 | 42.75 | 1.80 | 1.90 |
| CEM IgLMP-1(A) | 51.41 | 47.94 | 0.65 | 1.91 |
| CEM IgLMP-1(B) | 59.87 | 36.74 | 3.40 | 1.81 |
| CEM IgLMP-1(C) | 51.57 | 45.26 | 3.17 | 1.87 |
a
Cell cycles were synchronized by incubating cells for 12 h in serum-free medium and then for 24 h in culture medium before analysis. Cells were analyzed for cell cycle stage after staining with PI by using a flow cytometer and Cellfit software. Note the accumulation of LMP-expressing BJA-B cells, but not of LMP-expressing CEM cells, in the G2/M phase.