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. 2006 Jan 13;393(Pt 3):679–685. doi: 10.1042/BJ20051063

Figure 1. HES-related proteins and Ptf1-p48 co-immunoprecipitate in COS7 cell lysates.

Figure 1

(A) Association between Ptf1-p48 and Hes1. COS7 cells were transfected with expression vectors encoding V5 epitope-tagged Ptf1-p48 and FLAG-tagged Hes1, either alone or in combination. Immunoblot analysis of cell lysates prior to IP (immunoprecipitation) confirms appropriate expression following transient transfection. Following IP with the anti-V5 antibody, FLAG–Hes1 was detectable in the immunoprecipitate only in the presence of V5–Ptf1-p48. (B) Association of Ptf1-p48 with Hey1 and Hey2. COS7 cells were transfected with expression vectors encoding Ptf1-p48, FLAG–Hey1 and FLAG–Hey2, either alone or in combination. Pre-IP samples confirmed appropriate expression. Following IP with the anti-FLAG antibody, immunoreactive Ptf1 was present in the immunoprecipitate only in the presence of either Hey1 or Hey2. Pre-IP samples represented one-tenth of the total cell lysates used in the IP reaction.

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