Figure 2. Hes1 and the C-terminal domain of Ptf1-p48 interact in a yeast two-hybrid assay.

Colonies from SD (synthetic drop-out) media lacking leucine and tryptophan were streaked on X-Gal-containing SD plates lacking leucine, tryptophan and histidine. Vectors containing the Gal4 DNA-binding domain in-frame with either the C-terminal domain of Ptf1-p48 (pGBK-Ptf1 C-terminal) or with a combined bHLH/C-terminal domain (pGBK-Ptf1 bHLH+C-terminal) did not produce viable colonies when combined with an empty Gal4 activation vector (pGAD), but did produce blue colonies when combined with the Gal4 activation domain in-frame with Hes1. No viable colonies were observed when pGAD-Hes1 was transformed with an empty pGBK vector.