Table 1. Synthetic oligonucleotides used for site-directed mutagenesis.

The mutated nucleotides are underlined and in bold.

Mutant (forward)	Primer sequence (5'–3')
S258A	GTGCAGATCAACAGAGCCCCGTATTCTTACCTTC
S354A	AACCTAGGAGATGTGGCCCCCAACATTCTTGGA
S374A	TGTGATAACGCCAATGCCACTTGTCCCATTGGT
S396A	CAGGATATGTTTGACGCCACACAAATTATAGGA
S595A	CGATATGAGGCAGCAGCCACAATTTATGGACCG
S729A	GGACTCCTGGGTCTGGCCAATGCAACAGTGGAA
D352S	TCATCAAACCTAGGAGGTGTGTCCCCCAACATT
C362A	ATTCTTGGACCACGTGCCATCAACACAGGAGAG