Table 1. Effect of different compounds on the α- and β-NAD⁺_e-induced Ca²⁺ response of human granulocytes.

Before exposure to 100 μM α- or β-NAD⁺, granulocytes were pre-incubated at 25 °C in HBSS under the following conditions: with 100 μM 8-Br-cADPR or 50 μM ryanodine for 90 min; with 10 μM SKF96365 for 1 min; with 5 μM U73122 for 10 min; with 2 μM staurosporine for 60 min; or with 1 μM PKA inhibitor for 15 min. The [Ca²⁺]_i and the [cADPR]_i were measured 10 and 15 min respectively after exposure to NAD⁺. Results are expressed as percentages of the inhibition of α- or β-NAD⁺-induced responses in control cells, not pre-incubated with the inhibitors. Each value is the mean±S.D. for at least three different experiments. n.d., not determined.

	[Ca2+]i (%)		[cADPR]i (%)
Inhibitor	α-NAD+	β-NAD+	α-NAD+
8-Br-cADPR	80±6	75±5	n.d.
Ryanodine	81±6	77±5	n.d.
SKF96365	75±4	70±8	n.d.
U73122	0	0	n.d.
Staurosporine	75±6	78±7	90±5
PKA inhibitor	55±7	52±8	65±6