Figure 1. Isolation of neuropeptides from corpora cardiaca of T. fascicularis.

Analysis was performed on a Nucleosil 100 C-18 column eluted with a linear gradient of 0.11% (v/v) TFA (solvent A) and 0.1% (v/v) TFA in 60% acetonitrile (solvent B). The gradient ran from 33 to 53% B within 40 min at a flow rate of 1 ml·min⁻¹. A methanolic extract of 10 pairs of corpora cardiaca from T. fascicularis was injected and (A) UV absorbance at 214 nm and (B) fluorescence at 276 nm (excitation) and 350 nm (emission) were recorded.