Table 3. Hypertrehalosaemic and hyperprolinaemic activity of synthetic Trifa-CC from T. fascicularis, a non-phosphorylated form of Trifa-CC and the locust neuropeptide Locmi-HrTH in the protea beetle.
Protea beetles, T. fascicularis, of both genders were injected with 10 μl of the indicated substance, and haemolymph concentrations of total carbohydrates, proline and alanine were determined. *, 2% solvent B (as described in the Experimental section). Values are means±S.D., the significance of the difference between values before and after injection is indicated by †P<0.05 (paired Student's t test); whereas the significance of the difference from the control injection is indicated by ‡P<0.05 (A11A with post-hoc Dunnett test).
| Concentration in haemolymph | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Carbohydrates (mg·ml−1) | Proline (μmol·ml−1) | Alanine (μmol·ml−1) | ||||||||
| Treatment | n | 0 min | 90 min | Concn. change | 0 min | 90 min | Concn. change | 0 min | 90 min | Concn. change |
| Control injection* | 11 | 10.5±5.4 | 10.4±5.2 | −0.1±0.7 | 90.2±6.0 | 90.2±5.5 | 0±1.5 | 4.5±2.2 | 4.5±2.1 | 0±0.5 |
| Trifa-CC (phosphorylated) | ||||||||||
| 10 pmol | 11 | 15.3±6.5 | 21.0±12.2 | 5.7±7.9†‡ | 92.0±14.5 | 110.3±11.6 | 18.3±8.0†‡ | 4.1±1.6 | 1.1±0.5 | −3.0±1.6†‡ |
| 2 pmol | 7 | 10.5±4.2 | 16.6±6.2 | 6.1±2.5†‡ | 89.2±10.4 | 95.9±10.0 | 6.7±2.0†‡ | 4.6±2.5 | 2.2±0.9 | −2.4±1.9†‡ |
| Trifa-CC (non-phosphorylated) | ||||||||||
| 10 pmol | 11 | 11.0±4.2 | 13.9±6.8 | 2.9±4.1†‡ | 90.2±10.2 | 101.9±10.1 | 11.7±6.4†‡ | 5.0±3.8 | 2.4±1.6 | −2.6±2.7†‡ |
| 2 pmol | 7 | 12.3±4.7 | 15.6±3.1 | 3.3±5.4 | 90.0±11.7 | 95.3±9.7 | 5.3±2.9† | 4.2±1.3 | 2.6±1.0 | −1.6±0.6† |
| Locmi-HrTH | ||||||||||
| 100 pmol | 8 | 4.6±3.0 | 4.7±3.6 | 0.1±1.8 | 91.2±10.2 | 94.3±11.8 | 3.1±3.1† | 4.5±1.4 | 3.0±1.0 | −1.5±1.0† |
| 10 pmol | 7 | 14.0±3.1 | 15.0±3.3 | 1.0±2.1 | 91.1±9.9 | 93.3±8.5 | 2.2±4.3 | 3.9±1.3 | 3.5±1.0 | −0.4±1.5 |