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. 2006 Jan 13;393(Pt 3):705–713. doi: 10.1042/BJ20050735

Table 3. Hypertrehalosaemic and hyperprolinaemic activity of synthetic Trifa-CC from T. fascicularis, a non-phosphorylated form of Trifa-CC and the locust neuropeptide Locmi-HrTH in the protea beetle.

Protea beetles, T. fascicularis, of both genders were injected with 10 μl of the indicated substance, and haemolymph concentrations of total carbohydrates, proline and alanine were determined. *, 2% solvent B (as described in the Experimental section). Values are means±S.D., the significance of the difference between values before and after injection is indicated by †P<0.05 (paired Student's t test); whereas the significance of the difference from the control injection is indicated by ‡P<0.05 (A11A with post-hoc Dunnett test).

Concentration in haemolymph
Carbohydrates (mg·ml−1) Proline (μmol·ml−1) Alanine (μmol·ml−1)
Treatment n 0 min 90 min Concn. change 0 min 90 min Concn. change 0 min 90 min Concn. change
Control injection* 11 10.5±5.4 10.4±5.2 −0.1±0.7 90.2±6.0 90.2±5.5 0±1.5 4.5±2.2 4.5±2.1 0±0.5
Trifa-CC (phosphorylated)
 10 pmol 11 15.3±6.5 21.0±12.2 5.7±7.9†‡ 92.0±14.5 110.3±11.6 18.3±8.0†‡ 4.1±1.6 1.1±0.5 −3.0±1.6†‡
 2 pmol 7 10.5±4.2 16.6±6.2 6.1±2.5†‡ 89.2±10.4 95.9±10.0 6.7±2.0†‡ 4.6±2.5 2.2±0.9 −2.4±1.9†‡
Trifa-CC (non-phosphorylated)
 10 pmol 11 11.0±4.2 13.9±6.8 2.9±4.1†‡ 90.2±10.2 101.9±10.1 11.7±6.4†‡ 5.0±3.8 2.4±1.6 −2.6±2.7†‡
 2 pmol 7 12.3±4.7 15.6±3.1 3.3±5.4 90.0±11.7 95.3±9.7 5.3±2.9† 4.2±1.3 2.6±1.0 −1.6±0.6†
Locmi-HrTH
 100 pmol 8 4.6±3.0 4.7±3.6 0.1±1.8 91.2±10.2 94.3±11.8 3.1±3.1† 4.5±1.4 3.0±1.0 −1.5±1.0†
 10 pmol 7 14.0±3.1 15.0±3.3 1.0±2.1 91.1±9.9 93.3±8.5 2.2±4.3 3.9±1.3 3.5±1.0 −0.4±1.5