Table 3. Hypertrehalosaemic and hyperprolinaemic activity of synthetic Trifa-CC from T. fascicularis, a non-phosphorylated form of Trifa-CC and the locust neuropeptide Locmi-HrTH in the protea beetle.
Concentration in haemolymph | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
Carbohydrates (mg·ml−1) | Proline (μmol·ml−1) | Alanine (μmol·ml−1) | ||||||||
Treatment | n | 0 min | 90 min | Concn. change | 0 min | 90 min | Concn. change | 0 min | 90 min | Concn. change |
Control injection* | 11 | 10.5±5.4 | 10.4±5.2 | −0.1±0.7 | 90.2±6.0 | 90.2±5.5 | 0±1.5 | 4.5±2.2 | 4.5±2.1 | 0±0.5 |
Trifa-CC (phosphorylated) | ||||||||||
10 pmol | 11 | 15.3±6.5 | 21.0±12.2 | 5.7±7.9†‡ | 92.0±14.5 | 110.3±11.6 | 18.3±8.0†‡ | 4.1±1.6 | 1.1±0.5 | −3.0±1.6†‡ |
2 pmol | 7 | 10.5±4.2 | 16.6±6.2 | 6.1±2.5†‡ | 89.2±10.4 | 95.9±10.0 | 6.7±2.0†‡ | 4.6±2.5 | 2.2±0.9 | −2.4±1.9†‡ |
Trifa-CC (non-phosphorylated) | ||||||||||
10 pmol | 11 | 11.0±4.2 | 13.9±6.8 | 2.9±4.1†‡ | 90.2±10.2 | 101.9±10.1 | 11.7±6.4†‡ | 5.0±3.8 | 2.4±1.6 | −2.6±2.7†‡ |
2 pmol | 7 | 12.3±4.7 | 15.6±3.1 | 3.3±5.4 | 90.0±11.7 | 95.3±9.7 | 5.3±2.9† | 4.2±1.3 | 2.6±1.0 | −1.6±0.6† |
Locmi-HrTH | ||||||||||
100 pmol | 8 | 4.6±3.0 | 4.7±3.6 | 0.1±1.8 | 91.2±10.2 | 94.3±11.8 | 3.1±3.1† | 4.5±1.4 | 3.0±1.0 | −1.5±1.0† |
10 pmol | 7 | 14.0±3.1 | 15.0±3.3 | 1.0±2.1 | 91.1±9.9 | 93.3±8.5 | 2.2±4.3 | 3.9±1.3 | 3.5±1.0 | −0.4±1.5 |