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. 2006 Jan 13;393(Pt 3):767–777. doi: 10.1042/BJ20050780

Figure 2. Complementation analysis of E. coli LJ14 with RRFs.

Figure 2

The E. coli LJ14 (frrts) strain was transformed with the respective plasmids and examined for growth-phenotype. (A) Growth curves of various transformants of E. coli LJ14 (frrts) at the permissive temperature (30 °C). Vector, transformants with pQE-60; EcoRRF, transformants with pQE-EcoRRF; EcoDI/TteDII, transformants with pQE-EcoDI/TteDII; TteDI/DII, transformants with pQE-TteDI/EcoDII; TteRRF, transformants with pQE-TteRRF. (B) Growth curves of various transformants of E. coli LJ14 (frrts) at the non-permissive temperature (42 °C). The transformants are the same as those indicated in (A). (C) Detection of RRF expression in E. coli LJ14 by immunoblotting using anti-EcoRRF antibodies. Protein extracts were prepared from transformants harbouring the respective plasmids. Bands: 1, pQE-60 vector; 2, PQE-EcoRRF; 3, pQE-TteRRF; 4, pQE-EcoDI/TteDII; 5, pQE-TteDI/EcoDII. (D) Growth curves of various transformants of E. coli LJ14 (frrts) at the non-permissive temperature (42 °C). Vectors, transformants with pQE-60 and pSTV-28; TteEF-G, transformants with pQE-60 and pSTV-TteEF-G; TteRRF, transformants with pQE-TteRRF and pSTV-28; TteRRF and Tte-EFG, transformants with pQE-TteRRF and pSTV-TteEF-G. (E) Growth curves of various transformants of E. coli LJ14 (frrts) at the non-permissive temperature (42 °C). Vectors, transformants with pQE-60 and pSTV-28; Tte-EFG, transformants with pQE-60 and pSTV-TteEF-G; EcoDI/TteDII, transformants with pQE-EcoDI/TteDII and pSTV-28; EcoDI/TteDII&Tte-EFG, transformants with pQE-EcoDI/TteDII and pSTV-TteEF-G. The experimental conditions are described in the text.