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. 2006 Feb 2;34(3):773–784. doi: 10.1093/nar/gkj465

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© The Author 2006. Published by Oxford University Press. All rights reserved

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Dicer activity is not enhanced in the helF knock-out strain. Dicer activity in cell free extracts of wild-type and helF knock-out strains was examined as described previously (36). A 600 bp dsRNA generated from in vitro transcripts of the discoidin gene was used as a substrate and increasing amounts of extract (1, 5, 10 and 20 µg of protein as determined by Bradford assay) were added. Incubation was for 60 min at room temperature. The dsRNA substrate and the 21mer products are indicated. The marker represents end-labelled DNA fragments (pGEM-3Z digested with Sau3A).