FIG. 3.

Analysis of the effects of Nef on cell proliferation. Jurkat, B5 uninduced, and B5 induced cells were labeled with CFSE and cultured for 5 days. Cells were harvested, and fluorescence intensity and cell counts were analyzed by flow cytometry. Proliferation and cell division were characterized by sequential reduction in CFSE fluorescence intensity, generating a series of shifted equally spaced peaks on a logarithmic scale. (A) Comparison of CFSE intensity profiles of freshly labeled Jurkat cells (left) and cells after 5 days of culture (right). (B) Histograms showing different discrete cycles of cell division. Shown are cell division cycles of Jurkat and B5 uninduced cells (top) and B5 induced cells (bottom) after 5 days of culture.