FIG. 6.

Expression of cell cycle regulatory proteins in Jurkat and Nef-expressing cells. (A) Analysis of cyclin expression. Exponentially growing Jurkat, B5 uninduced, and B5 induced cells were subjected to Western blot analysis using 20 μg of protein for each cell line. The cyclin D1, cyclin A, and cyclin E proteins were detected by Western blot analysis. Membranes were reblotted with actin antibodies to ensure equal loading of the samples. (B) Analysis of CKI expression. Cells were subjected to Western blot analysis using 20 μg of protein for each cell line. Samples were analyzed on an SDS-12 (p21) or 10% (p27) PAGE gel. (C) Analysis of Rb family and E2F expression. Cells were subjected to Western blot analysis using 40 μg of protein. Samples were analyzed on an SDS-8 (Rb, hyperphosphorylated Rb, p130, and p107) or 10% (E2F1 and E2F4) PAGE gel.