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Identification and characterization of BHV-5 Us9 ORF-specific proteins with a rabbit antibody to 14-mer BHV-5 Us9-specific polypeptides. Immunoblot analysis of mock-infected, BHV-5Us9Δ-, and BHV-5-infected cell lysates (A); mock-infected cell lysates and partially purified BHV-5 and BHV-5Us9Δ virion lysates (B); and BHV-5 polypeptides produced in untreated (PAA−) and PAA-treated (PAA+) BHV-5-infected cell lysates (C). (C) Western blot of an SDS-12% polyacrylamide gel.
