FIG. 4.

Percentage of HDV RNA cDNA clones with non-amber/W A → G changes in the HDAg coding region (nucleotides 949 to 1598). The percentage of clones (represented on the y axis) with the indicated number of non-amber/W changes (represented on the x axis) is shown. As described in Materials and Methods, the cDNA populations were derived, by RT-PCR, from replicating HDV RNAs harvested from Huh7 human hepatoma cells 4 days after cotransfection with 1 μg each of the replicating HDV RNA expression construct (pHDV), designed to synthesize antigenomic HDV RNA, and either an ADAR1 (pAD1) or ADAR2 (pAD2) expression construct. Fifty cDNA clones each were analyzed for ADAR1 and ADAR2.