FIG. 1.

Analysis of HDV RNA-transfected cells by metabolic labeling and Northern blotting. (A) ³²P-labeled RNA from antigenomic HDV RNA-transfected HuH7 cells. RNA was separated by electrophoresis on a 1.2% denaturing agarose gel. Lane 1, 1.75-kb RNA marker; lanes 2 and 3, mock-transfected cells; lanes 4 to 6, HDV RNA-transfected cells. Each lane represents independently transfected cell cultures. 1x and 2x, positions of monomer and dimer HDV RNAs, respectively. The 28S and 18S rRNA species are also labeled. (B) Northern blot analysis. HuH7 cells transfected with either genomic (HDV-G) or antigenomic (HDV-AG) HDV RNA were blotted for the detection of genomic HDV RNA at 4 days after transfection. Lane δ9, genomic HDV RNA marker (25). 3x, position of trimer HDV RNA.