FIG. 3.

Monomeric HDV RNA is derived from the processing of higher-molecular-weight species. (A) ³²P-labeled RNA from antigenomic HDV RNA-transfected cells, metabolically labeled for 30 to 240 min. The duration of labeling and amount of RNA loaded in each lane are indicated at the top and bottom of each track, respectively. The mock-transfected HuH7 cells were labeled for 240 min. 1x and 2x, monomer and dimer HDV RNA, respectively. (B) ³²P-labeled RNA from mock- (panel 4) and HDV RNA-transfected (panel 5) HuH7 cells was reverse hybridized with membranes containing unlabeled RNA probes specific for genomic (G) and antigenomic (AG) HDV RNA. Cell, probe consisting of total cellular RNA extracted from untransfected cells. The specificity controls shown in panels 1 to 3 are reverse hybridizations with unlabeled HuH7 cell RNA spiked with [³²P]orthophosphate-labeled (20,000 cpm) full-length genomic or antigenomic HDV RNA or a 50:50 mixture of both (G + AG) to the membranes as described above.