FIG. 5.

Sensitivity of genomic HDV RNA synthesis to α-amanitin analyzed with steady-state RNA. HuH7 cells were transfected with 1.9-kb antigenomic HDV RNA and mRNA encoding S-HDAg and treated with α-amanitin (5 μg/ml) at various time points posttransfection. Total RNA was extracted 2 days after treatment and subjected to Northern blot analysis using a ³²P-labeled antigenomic-sense riboprobe. Lane 1, positive control marking the position of the 1.7-kb genome; lanes 3 to 7, total RNA harvested from treated and untreated transfected cells at the indicated time points. Cells were treated for 48 h prior to harvest. For example, in lane 3, cells were treated with α-amanitin at day 1 and harvested at day 3 posttransfection.