FIG. 1.

Identification of MHV ORF1a products recognized by anti-D14 serum. (A) Detection of p150 and p22 ORF1a proteolytic products. HeLa-MHVR cells were infected with MHV A59, and newly synthesized proteins were labeled with Trans³⁵S-label from 3.5 to 5.5 hpi. Total lysates of uninfected (U) or infected (I) cells were subjected to immunoprecipitation with preimmune (Pre) or anti-D14 serum. Products were analyzed by electrophoresis on an SDS-5.0 to 12.5% polyacrylamide gel and subjected to autoradiography. (B) Pulse-chase analysis of MHV JHM-x-infected cells. Proteins were pulse-labeled with Trans³⁵S-label from 4.5 to 5.0 hpi. Labeling medium was then replaced with complete medium containing 10× methionine and cysteine. Cells were lysed at the chase times indicated, and total lysates were subjected to immunoprecipitation with anti-D14 serum. Products were analyzed on an SDS-7.5 to 12.5% polyacrylamide gel and subjected to autoradiography. Sizes are shown on the left in kilodaltons.