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Detection of newly synthesized viral RNA in MHV-infected HeLa-MHVR cells at 5 hpi. MHV-infected cells were incubated with BrUTP in the presence of actinomycin D for 1 h to allow incorporation of label into newly synthesized MHV RNA. BrU-RNA was detected using an anti-BrdU antibody and goat anti-mouse Ig antibody coupled to 10-nm gold particles. The BrU-RNA was detected in association with the DMVs. Bar, 200 nm.
