FIG. 2.

Transcription of ASFV IAP gene in Jurkat cells stably transfected with A224L. RNAs were isolated from a pool of three different clones of A224L-transfected (lane 2) or pcDNA-transfected (lane 3) Jurkat cells. PCR products were generated using A224L-specific primers, and cDNAs were synthesized from RNAs and resolved in a 1.5% agarose gel. A glyceraldehyde-3-phosphate dehydrogenase (GAPDH) control was used to control the RNA content and integrity. Positions of molecular size markers are included, and the sizes (in kilobase pairs) are indicated on the left.