FIG. 5.

Involvement of IKKs in ASFV IAP-mediated NF-κB activity. pcDNA3-transfected or A224L stably expressing Jurkat cells were cotransfected with 1 μg of the NF-κB-dependent promoter-luciferase reporter gene together with 1 μg of pRK5 empty plasmid or pRK5 IKKβ. After 24 h of culture, cells were either untreated or stimulated with TNF-α (50 ng/ml) for 16 h. The specificity of the effect was ensured by transfection of 1 μg of a dominant negative mutant of the IKKβ expression vector in pcDNA3-expressing cells or A224L-expressing cells. Relative luciferase values (RLU) per microgram are averages (mean ± SE) of two representative experiments in which each transfection was done in triplicate.