FIG. 1.
Effects of insulin and IGF-1 on apoptosis in ts13. A total of 105 ts13 cells/well were grown overnight in 12-well plates at 32°C. The medium was then changed to 0.5% FCS-DMEM and placed at either 32°C or 39°C. For growth factor treatment, the medium was supplemented with 100 ng of insulin or IGF-1 per ml. To inhibit PI3 kinase, 20 μM LY294002 (LY) was used as described in Materials and Methods. After 20 h at 39°C, the cells were harvested, and apoptosis was analyzed using the cell death detection ELISA as described in Materials and Methods.