FIG. 2.
Colocalization of SMN, p80 coilin, SIP1, and NS1 in transfected A92L cells. SMN (primary antibody: MANSMA1; secondary antibody: TRITC [red]), SIP1 (primary antibody: MANSIP1A; secondary antibody: anti-mouse TRITC conjugate [red]), p80 coilin (primary antibody: 5P10 monoclonal antibody; secondary: anti-mouse TRITC conjugate [red]), and NS1 (primary antibody: rabbit anti-NS1 polyclonal; secondary antibody: anti-rabbit FITC conjugate [green]) are indicated. Arrows, Cajal bodies containing NS1. Insets show nuclear regions of interest magnified by an additional factor of 2. Bar ≈ 30 μm.
