FIG. 2.
Calorimetric measurement of VSV binding to liposomes at 35oC. Typical calorimetric traces (heat flow as a function of time) obtained for four to eight injections (5 μl each) of a VSV suspension (28 μg/ml) into the cell containing unilamellar vesicles of PC-PE-PS with cholesterol (3:1:1 and 10%), PC-PS (1:3), or PC only, in 20 mM MES-30 mM Tris, pH 7.5, at 35°C. The sharp peaks are due to the VSV dilution, as seen in control experiments of the injection of virus into buffer (not shown). The phospholipid concentration was 1 mM.