FIG. 3.

SV5 F protein can replace HN for budding of particles. 293T cells were either infected with rSV5 or transfected with plasmids as indicated. Plasmid quantities are indicated in Materials and Methods, except that increasing HN and F plasmid amounts represent 1.0, 1.5, and 2.0 μg of plasmid DNA. Budding assays were carried out as described in the legend to Fig. 2. Budding efficiency was calculated as the fraction of total SV5 M protein detected in the culture media.