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Truncation of the HN cytoplasmic tail results in defective budding of both recombinant viruses and VLPs. 293T cells were either infected with the indicated viruses (A) or transfected with the indicated plasmids (B). Budding assays were carried out as described in the legend to Fig. 2. Budding efficiency was calculated as the fraction of total SV5 M protein detected in the culture media.
