FIG. 2.

LCMV-specific IFN-γ-producing CD8 T cells in LTα^−/− mice. On day 8 after infection with LCMV, splenocytes from +/+ and LTα^−/− mice were stimulated in vitro with CTL epitope peptides (NP396-404, GP33-41, or GP276-285) for 5 h. The number of LCMV-specific CD8 T cells was determined by intracellular staining for IFN-γ. (a) Flow cytometry profiles of staining for IFN-γ following stimulation with specific peptides. Percentages of IFN-γ-producing CD8 T cells among splenocytes are given. (b) Total numbers of IFN-γ-producing CD8 T cells in the spleens of +/+ and LTα^−/− mice. Note that the CD8 T-cell responses to NP396-404, GP33-41, and GP276-285 were 54-, 17-, and 7-fold lower, respectively, in LTα^−/− mice than in +/+ mice.