FIG. 8.

LCMV-specific IFN-γ-producing CD8 and CD4 T cells in mice receiving adoptive transfer. Irradiated congenic B6.PL-Thy1^a/Cy (Thy-1.1) mice received 10 × 10⁷ spleen cells from +/+ or LTα^−/− mice (designated +/+ donors and LTα^−/− donors, respectively). Eight days after infection with LCMV, virus-specific CD8 and CD4 T cells in the spleens of mice receiving spleen cells from +/+ donors and LTα^−/− donors were quantitated by intracellular cytokine staining as described in the legends to Fig. 2 and 4. Costaining with anti-Thy1.2 antibodies showed that all of the IFN-γ-producing T cells were of donor origin. Panels a and b show the total numbers of LCMV-specific CD8 and CD4 T cells, respectively, in the spleens of mice receiving spleen cells from +/+ donors and LTα^−/− donors. Data are means for four mice per group.