TABLE 2.

Sequences of DSCEs in rescued plasmids^a

Construct	Type of cell population/(no. of sequences)	nt	No. of sequences with nt at position:
			1234	1235	1236	1237	1238	1239
pF1fsdsGFPran1	Unsorted (90)	T	[29]	34	27	28	35	30
		C	21	14	23	[21]	[18]	16
		G	28	[28]	[26]	32	26	32
		A	12	14	14	9	11	[12]
	Sorted (91)	T	[77]	14	5	11	12	21
		C	2	3	1	[59]	[72]	12
		G	7	[72]	[84]	21	2	5
		A	5	2	1	0	5	[53]
pF1fsdsGFPran2	Unsorted (91)	T	40	34	31	37	36	[34]
		C	17	[16]	[13]	24	17	9
		G	20	24	27	[14]	[26]	31
		A	[14]	17	20	16	12	17
	Sorted (87)	T	19	12	17	11	28	[44]
		C	11	[65]	[57]	27	10	6
		G	18	2	10	[45]	[32]	30
		A	[39]	8	3	4	17	7

^aPlasmid DNA was recovered from yeast cell populations, prior to or after sorting by fluorescence-activated cell sorter, and isolated in bacterial host DH5α. The sequences corresponding to FHV RNA positions 1234 to 1239 were determined for 96 independent plasmid preparations from each starting population. Only those sequences that were free of ambiguities in this region were tabulated, and their numbers are shown in parentheses. The most abundant nucleotide at each position is in boldface, and the numbers of sequences complementary to positions 2515 to 2520 are in brackets.