FIG. 1.

RNA patterns of five alstroemeria-infecting CMV strains (ALS-0 to -4). Northern blot analysis used a mixture of two digoxigenin-labeled cDNA probes specific for the RNA 2-derived 218 nt in RNA 3 of ALS-1 and ALS-2 (10) and for the CP ORF of subgroup II CMV. The CP-specific probe was PCR amplified with a pair of degenerate primers corresponding to positions 1227 to 1240 and 1866 to 1883 on RNA 3 of ALS-0. A subgroup II isolate from Crocus (9) was used as a wild-type (wt) reference isolate. Size ranges of the genomic RNAs (and subgenomic RNA 4) are indicated on the left.