FIG. 3.

Agarose gel electrophoresis of RT-PCR fragments amplified from total RNA of tobacco or alstroemeria leaves infected with CMV isolates ALS-0 and ALS-2 in various ratios. The degenerate primers used amplified the intercistronic region, CP ORF, and 3′ NTR of RNA 3, resulting in cDNA fragments of 1.1 kb (ALS-0; wild type) or 1.3 kb (ALS-2) (9). Lambda DNA digested with PstI was used as a size marker (M).