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Induction of IFN-β mRNA after VVΔE3L infection. HT-1080 cells were infected with either wild-type VV, VVΔE3L, or reconstituted wild-type VV (WR strain; MOI of 15) for the lengths of time indicated. The expression of IFN-β (upper panel) or GAPDH mRNA (middle panel) was detected by RT-PCR analysis. The products were analyzed on polyacrylamide gels and by autoradiography. Ethidium bromide staining was performed on the total RNA (lower panel).
