FMD	foot-and-mouth disease
WOAH	World Organisation for Animal Health
FMDV	foot-and-mouth disease virus
RNA	ribonucleic acid
WRLFMD	World Reference Laboratory for Foot-and-Mouth Disease
BHK T7-9	baby hamster kidney cells expressing T7 RNA polymerase
LF-BK	fetal porcine kidney cells
DMEM	Dulbecco’s Modified Eagle Medium
DNA	deoxyribonucleic acid
BSL-3	biosafety level 3
PCR	polymerase chain reaction
BEI	binary ethyleneimine
TEM	transmission electron microscopy
2D-VNT	two-dimensional virus neutralization test
dpv	days post-vaccination
CPE	cytopathic effect
TCID50	50% tissue culture infectious dose
dpc	days post-challenge
ELISA	enzyme-linked immunosorbent assay
S/N	sample-to-negative control
RT-PCR	real-time reverse transcription polymerase chain reaction
VN	virus neutralization
VNT	virus neutralization testing
VP 1-4	viral proteins 1-4
MEGA	molecular evolutionary genetics analysis
NC	negative control
Comm. Vac.	commercial vaccine
r1	antigenic relationship value
IACUC	Institutional Animal Care and Use Committee
APQA	Animal and Plant Quarantine Agency
SEM	standard error of mean
ANOVA	analysis of variance
PAN	pan-serotype test line