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. 2002 May;76(10):4688–4698. doi: 10.1128/JVI.76.10.4688-4698.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 3. — Lytic expression of vOX2. (A) Flow cytometry analysis of vOX2 surface expression. Unstimulated JSC-1 or TPA-stimulated JSC-1 cells were reacted with an anti-vOX2 antibody, followed by FITC-conjugated anti-mouse immunoglobulin G. As a negative control, unstimulated JSC-1 cells were stained with FITC conjugated anti-mouse immunoglobulin G. Percentages are vOX2-positive cells. (B) vOX2 expression during to KSHV lytic replication. Unstimulated JSC-1, TPA-stimulated JSC-1 cells, or BJAB cell lysates were separated by SDS-PAGE, transferred to nitrocellulose membrane, and reacted with mouse anti-vOX2 antibody, rabbit anti-vIRF antibody, rabbit anti-K8.1 antibody, rabbit anti-MIR1 antibody, and rabbit anti-LANA antibody. As an internal control, donkey antiactin antibody was used.