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HPV18/16 chimeric recombinant plasmid. Speckled areas represent HPV18 early genes and the URR. The striped area represents HPV16 late genes. The approximate positions of the HPV ORFs are shown in insets. White areas represent the pBSSK(+) vector; the position of the Ampr gene is shown. HPV18 was cloned into pBSSK(+) at the unique EcoRI site. The HPV18 late gene ORFs were removed and replaced with a unique BglII site. The HPV16 late gene ORFs were inserted into this unique BglII site.
