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Subcellular location of SV40 at 5 h. CV-1 cells were exposed to SV40 at an MOI of 100 PFU per cell for 1 h at 4°C and then incubated for 5 h at 37°C. (A and D) Cells immunostained for SV40; (B) cells immunostained for β-COP; (E) cells immunostained for the ER marker PDI; (C) merge of panels A and B; (F) merge of panels D and E.
