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Effect of BFA on subcellular location of SV40 at 5 and 10 h. CV-1 cells were exposed to SV40 at an MOI of 100 PFU per cell for 1 h at 4°C and then incubated for 5 h (A, B, and C) or 10 h (D, E, and F) at 37°C. BFA (0.5 μg/ml) was added to the cell cultures after 30 min at 37°C and was present thereafter. (A and D) Cells immunostained for SV40; (B and E) cells immunostained for the ER marker PDI; (C) merge of panels A and B; (F) merge of panels D and E.
