Figure 3.
Effects of DHT on caspase activity in PC3-AR transfectants. Cells were plated on 96-well plates and cultured in SFC. Caspase activity was measured in living cells as described in the legend to Figure 1. Cells were treated with 10 pm, 10 nm and 10 μm of DHT together with TNF-α (20 ng/ml), anti-Fas mAb (100 ng/ml) or TRAIL (100 ng/ml). Substrate hydrolysis was measured after 48 h of treatment. PC-3(AR)2 cell line was obtained from Dr TJ Brown and marked here as PC3-AR-1