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. Author manuscript; available in PMC: 2006 Aug 1.
Published in final edited form as: FEBS J. 2005 Aug;272(16):4249–4259. doi: 10.1111/j.1742-4658.2005.04839.x

FIGURE 1.

FIGURE 1

Biotinylation targets amino acids in the N-terminal tail of human histone H3. Synthetic peptides based on the N- and C-terminal region in histone H3 were biotinylated enzymatically, and biotin was probed using gel electrophoresis and streptavidin peroxidase. N1–25 = peptide spanning amino acids 1–25 in histone H3 (lanes 1a and b); N15–39 = peptide spanning amino acids 15–39 in histone H3 (lanes 2a and b); C116–136 = peptide spanning amino acids 116–136 in histone H3 (lanes 3a and b). Duplicate analyses are depicted.