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. Author manuscript; available in PMC: 2006 Aug 1.
Published in final edited form as: FEBS J. 2005 Aug;272(16):4249–4259. doi: 10.1111/j.1742-4658.2005.04839.x

FIGURE 7.

FIGURE 7

Biotinylated histones H3 are present in extracts from human cell nuclei, but biotinylation is reduced in biotinidase- and holocarboxylase synthetase-deficient cells. Panel A: Histones were extracted from JAr cell nuclei and biotinylated histones H3 were titrated using antibodies against biotinylated K4, K9, and K18; equal amounts of recombinant (non-biotinylated) histone H3 were used as negative control. Panel B: Histones were extracted from biotinidase- and holocarboxylase synthetase-deficient human skin fibroblasts (lanes a and b, respectively); IMR-90 fibroblasts from a healthy human were used as a control (lane c). Histones were probed using an antibody to K18-biotinylated histone H3. Equal loading was confirmed by staining with coomassie blue (lanes d – f).