Table IV.
A comparative HPLC separation of free amino acid composition (based on identical retention time to standard references) and concentration in the fractions with molecular mass ≤10 kD of Ni-S and Ni-D foliage from young pecan seedling trees
| Amino Acid
|
Retention Time
|
Concentration X ± sda
|
|
|---|---|---|---|
| Ni-D | Ni-S | ||
| min | μm/mL | ||
| Gly | 3.64 | 30.83 ± 3.75a | 12.63 ± 0.93b |
| Ser/Asn | 3.86 | 3.10 ± 0.45a | 3.30 ± 0.17a |
| Asp | 4.47 | 0.20 ± 0.06a | 0.20 ± 0.06a |
| Gln | 5.20 | 0.20 ± 0.06a | 0.08 ± 0.02a |
| Ala/Thr | 5.57 | 0.10 ± 0.02a | 0.07 ± 0.03a |
| Glu | 7.08 | 0.03 ± 0.00b | 0.07 ± 0.01a |
| Cys/Lys | 7.51 | 0.20 ± 0.03a | 0.29 ± 0.01a |
| His | 8.56 | 0.06 ± 0.00b | 0.15 ± 0.03a |
| Pro | 10.39 | 0.00 ± 0.00a | 0.01 ± 0.00a |
| Arg | 12.75 | 0.15 ± 0.03a | 0.07 ± 0.03b |
| Val | 13.38 | 3.23 ± 0.64a | 1.03 ± 0.04b |
| Met | 14.05 | 0.08 ± 0.01a | 0.06 ± 0.02a |
| Tyr | 14.64 | 0.48 ± 0.09a | 0.23 ± 0.00b |
| Ile | 15.86 | 0.96 ± 0.21a | 0.35 ± 0.00b |
| Leu | 16.51 | 0.27 ± 0.09a | 0.04 ± 0.02a |
| Phe | 18.22 | 0.06 ± 0.00a | 0.07 ± 0.01a |
| Trp | 22.66 | 0.30 ± 0.03a | 0.14 ± 0.00b |
a
Means (±sd) followed by the same letter are not significantly different (t test; P ≤ 0.05).