Table III.
Effects of leaf aging on the activity of the Fd-Glu synthase promoter in transgenic tobacco plants, which harbor the GLU1 promoter of the Arabidopsis Fd-GOGAT fused to a GUS reporter gene (GLU1::GUS)
In vitro GUS activity was determined in two transgenic lines: GLU1∷GUS*1 and GLU1∷GUS*2.
| Tissues
|
Young Leaves and Roots
|
Old Leaves and Roots
|
||
|---|---|---|---|---|
| GLU1∷GUS*1 | GLU1∷GUS*2 | GLU1∷GUS*1 | GLU1∷GUS*2 | |
| GUS activity: nmol 4-MU mg−1 protein min−1 | ||||
| Leaves | ||||
| Mesophyll | 58.41 ± 4.90 | 14.10 ± 1.27 | 34.05 ± 3.2 | 7.86 ± 1.08 |
| Vein | 5.02 ± 0.42 | 4.55 ± 0.41 | 4.67 ± 0.42 | 4.01 ± 0.36 |
| Petiole | 2.93 ± 0.25 | 2.17 ± 0.20 | 2.71 ± 0.23 | 1.95 ± 0.18 |
| Roots | 1.22 ± 0.09 | 0.45 ± 0.05 | 1.16 ± 0.11 | 0.41 ± 0.04 |