Skip to main content
. 2006 Feb;140(2):512–527. doi: 10.1104/pp.105.074294

Table II.

Summary of molecular and phenotypical analysis of transgenic maize (T0 and T1 generations, respectively) containing the ZmMCM6 gene under control of the strong and constitutive maize UBI promoter in sense and AS orientation or fused to GFP

n.d., Not determined.

Construct Plant No. Functional Transgenesa Transgene Quantityb Plant Heightc Fertilityd
− Wild type (A188) − 100/25/0.68 160–190 +
Overexpression
UBIp:MCM6 (T0) SE1a ≥5 (+) 0.66 158 +
UBIp:MCM6 (T0) SE1b ≥5 (+) 0.33 185 +
UBIp:MCM6 (T0) SE1c ≥5 (+) 0.27 183 +
UBIp:MCM6 (T0) SE1d ≥5 (+) 0.26 172 +
UBIp:MCM6-GFP (T0) G1 1 (−) n.d. (150) +
UBIp:MCM6-GFP (T0) G2 2 (−) n.d. (100) +
UBIp:MCM6-GFP (T0) G3 2 (−) n.d. (60) +
UBIp:MCM6-GFP (T0) G4 1 (−) n.d. (60) +
UBIp:MCM6-GFP (T0) G5 2 (−) n.d. (96) +
Down-regulation
UBIp:MCM6-AS (T0) AS1 ≥3 (−) 0.63 175 +
UBIp:MCM6-AS (T0) AS2 ≥4 (−) 0.96 182 +
UBIp:MCM6-AS (T0) AS3 ≥7 (+) 0.39 130 FS
UBIp:MCM6-AS (T0) AS4a ≥5 (+) 0.73 83 FS/(MS)
UBIp:MCM6-AS (T0) AS4b ≥5 (+) 0.48 86 FS/(MS)
UBIp:MCM6-AS (T0) AS4c ≥5 (+) 0.36 58 FS/MS
UBIp:MCM6-AS (T0) AS5 ≥6 (+) 1.10 66 FS/MS
UBIp:MCM6-AS (T0) AS6 ≥5 (+) 0.42 68 FS/(MS)
UBIp:MCM6-AS (T0) AS7 ≥4 (−) 0.90 182 +
UBIp:MCM6-AS (T0) AS8a ≥4 (−) 0.55 161 +
UBIp:MCM6-AS (T0) AS8b ≥4 (−) 0.78 159 +
UBIp:MCM6-AS (T0) AS8c ≥4 (−) 0.96 175 +
UBIp:MCM6-AS (T0) AS9a ≥4 (−) 0.08 174 +
UBIp:MCM6-AS (T0) AS9b ≥4 (−) 1.10 158 +
UBIp:MCM6-AS (T0) AS10a ≥6 (−) 0.42 187 +
UBIp:MCM6-AS (T0) AS10b ≥6 (−) 0.49 169 +
[UBIp:MCM6-AS] (T1)e AS4a/1–20 − n.d. 155–185 +
[UBIp:MCM6-AS] (T1)e AS4b/1–22 − n.d. 155–185 +
[UBIp:MCM6-AS] (T1)e AS6/1 − n.d. 150 +
a

Number of transgene integration events, functional transgene integrations (+), and lack of functional transgene (−), respectively.

b

Quantification of relative sense and AS transcript amounts. Expression in immature wild-type tassels (10 mm; see also Fig. 4A) was set as 100%. Relative expression in wild-type roots was 25% and 0.68% in mature leaves. No distinction between sense and AS transcript levels.

c

Plant height in centimeters. G1 to 5 plants were generated in winter after a long tissue culture stage.

d

Fully developed and fertile cobs as well as tassels (+). FS, Female sterility; MS, male sterility; parentheses, tassels mostly sterile, development of little pollen.

e

Pollen of transgenic lines were back crossed to wild-type (A188) plants to generate T1 progeny. One selfed kernel was obtained from a tassel flower of plant AS6, which developed into a female spikelet.