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. 2026 Sep 14;2026:gigabyte188. doi: 10.46471/gigabyte.188
Reviewer name and names of any other individual's who aided in reviewer chao bian
Do you understand and agree to our policy of having open and named reviews, and having your review included with the published papers. (If no, please inform the editor that you cannot review this manuscript.) Yes
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Are all data available and do they match the descriptions in the paper? Yes
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Are the data and metadata consistent with relevant minimum information or reporting standards? See GigaDB checklists for examples <a href="http://gigadb.org/site/guide" target="_blank">http://gigadb.org/site/guide</a> Yes
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Is the data acquisition clear, complete and methodologically sound? Yes
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Is there sufficient detail in the methods and data-processing steps to allow reproduction? Yes
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Is there sufficient data validation and statistical analyses of data quality? Yes
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Is the validation suitable for this type of data? Yes
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Is there sufficient information for others to reuse this dataset or integrate it with other data? Yes
Additional Comments This study, entitled “The genome of the coral model sea anemone Exaiptasia diaphana (Aiptasia) strain F003”, reports a genome assembly of sea anemone. This assembly was used the ONT sequencing for assembling. The N50 of contigs and scaffolds are 76.47kb and 77.93kb. 29589 genes were identified. For the quality of this assembly, why did not use the hifiasm to assemble the sequences? These contigs are indeed fragment. Pure ONT sequencing will largely improve the extensibility of contigs. This fragmented contigs have large potential to be of high quality. In addition, the author may sequence the HIC data to cluster this assembly to be a chromosome genome. Annotation pipeline is also somewhat outdate. Braker2 or egapx(NCBI) are indeed recommended. How many BUSCO value for the protein set?
Any Additional Overall Comments to the Author This study, entitled “The genome of the coral model sea anemone Exaiptasia diaphana (Aiptasia) strain F003”, reports a genome assembly of sea anemone. This assembly was used the ONT sequencing for assembling. The N50 of contigs and scaffolds are 76.47kb and 77.93kb. 29589 genes were identified. For the quality of this assembly, why did not use the hifiasm to assemble the sequences? These contigs are indeed fragment. Pure ONT sequencing will largely improve the extensibility of contigs. This fragmented contigs have large potential to be of high quality. In addition, the author may sequence the HIC data to cluster this assembly to be a chromosome genome. Annotation pipeline is also somewhat outdate. Braker2 or egapx(NCBI) are indeed recommended. How many BUSCO value for the protein set?
Recommendation Major Revision