Figure 1.

Regulation of glyoxalase I and II protein levels and enzyme activities by zinc. Western blot of total leaf/shoot proteins from seedlings exposed to different concentrations of ZnCl₂ for 24 h is shown. A, B. juncea leaf proteins probed with anti-glyoxalase I antibodies. B, Rice shoot proteins probed with anti-glyoxalase II antibodies. The positions of the glyoxalase I and II proteins are marked with arrows. C and D, Histograms showing the activity of glyoxalase I (C) and glyoxalase II (D) enzymes in the selected transgenic tobacco lines exposed to 5 mm ZnCl₂ for 24 h. Data represent the mean of three replicates and of three independent experiments ±sd. WT, Wild type; GI, glyoxalase I; GII, glyoxalase II; GI + II, double transgenics. Note that zinc exposure enhances protein levels and enzyme activities for both glyoxalase I and glyoxalase II.