Figure 4.

Relative MG levels and the degree of oxidative stress as well as lipid peroxidation in transgenic tobacco plants. A, Accumulation of MG (μm g⁻¹ fresh weight). B, H₂O₂ (nmol g⁻¹ fresh weight; for measuring oxidative stress). C, MDA (nmol g⁻¹ fresh weight; to reflect the extent of lipid peroxidation) in wild-type (WT) and glyoxalase transgenic plants (GI, glyoxalase I; GII, glyoxalase II; and GI + II, double transgenics) in response to zinc. Second leaf from top of 1-month-old T₁ generation plants of each of these types was floated in 5 mm ZnCl₂ solution for 24 h. Data represent the mean of three replicates and of three independent experiments ±sd.