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. 2006 Feb;140(2):647–660. doi: 10.1104/pp.105.073916

Figure 2.

Figure 2.

Identification of 14-3-3 isoforms recognized by three mAbs generated from 14-3-3/G-box DNA-binding complexes using 13 recombinant Arabidopsis 14-3-3 proteins. A, GBC mAbs detect a subset of nondenatured recombinant Arabidopsis 14-3-3 proteins. Samples of each purified recombinant Arabidopsis 14-3-3 protein were transferred to nitrocellulose with a dot-blot apparatus. After blocking, the blot was probed with GBC mAb 2A3, 4B9, and 5D6. Cross-reactivity was detected with anti-mouse horseradish peroxidase conjugate and chemiluminescence. B, GBC mAbs detect a subset of denatured recombinant Arabidopsis 14-3-3 proteins. Aliquots of 10 μg of each recombinant Arabidopsis 14-3-3 proteins were denatured in reducing SDS sample buffer prior to electrophoresis. After transferring electrophoresed proteins to nitrocellulose and blocking, the blot was probed with GBC mAb 2A3, 4B9, and 5D6. Immuno-cross-reactivity was detected with anti-mouse horseradish peroxidase conjugate and chemiluminescence.