Figure 8.

The epitope loop regions of 14-3-3s are the most variable structural elements between plant and animal 14-3-3s. The three-dimensional structures of a human (PDB accession no. 1IB1) and tobacco 14-3-3 (PDB accession no. 109C), an isoform with a medial Gly in the divalent cation-binding domain, were superimposed for identification of homologous and dissimilar structural elements using SuperPose 1.0 (Maiti et al., 2004). The difference distance plot identified several regions of the 14-3-3s that were structurally divergent at distances between 12 to 1.5 angstroms (Å). The GBC mAb epitopes mapped to three such regions, L5, L6, and L8.